PrimePCR™ Probe Assay: WISP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WISP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WISP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039113
Assay Design:   exonic
Chromosome Location:   8:134239844-134239945question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000250160 ENST00000519433 ENST00000517423 ENST00000220856 ENST00000377863

Gene Information

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain von Willebrand factor type C module thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq Mar 2011]

Gene Symbol:   WISP1
Gene Name:   WNT1 inducible signaling pathway protein 1
Aliases:   CCN4, FLJ14388, WISP1c, WISP1i, WISP1tc
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000104415
Entrez:   8840
Chromosome Mapping:   8q24.22

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