PrimePCR™ Probe Assay: GADD45A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GADD45A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GADD45A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039165
Assay Design:   exonic
Chromosome Location:   1:68153379-68153479question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000370986 ENST00000370985

Gene Information

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq Dec 2010]

Gene Symbol:   GADD45A
Gene Name:   growth arrest and DNA-damage-inducible, alpha
Aliases:   DDIT1, GADD45
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116717
Entrez:   1647
Chromosome Mapping:   1p31.2

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