PrimePCR™ Probe Assay: CNTFR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CNTFR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CNTFR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039204
Assay Design:   exonic
Chromosome Location:   9:34557524-34557625question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000351266 ENST00000378980 ENST00000417345

Gene Information

This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor which plays a critical role in neuronal cell survival differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor gp130 and leukemia inhibitory factor receptor facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq May 2011]

Gene Symbol:   CNTFR
Gene Name:   ciliary neurotrophic factor receptor
Aliases:   MGC1774
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000122756
Entrez:   1271
Chromosome Mapping:   9p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.910000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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