PrimePCR™ Probe Assay: TNFSF14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNFSF14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNFSF14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039220
Assay Design:   exonic
Chromosome Location:   19:6664884-6664986question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000245912 ENST00000599359 ENST00000326176

Gene Information

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14 which is a member of the tumor necrosis factor receptor superfamily and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   TNFSF14
Gene Name:   tumor necrosis factor (ligand) superfamily, member 14
Aliases:   CD258, HVEML, LIGHT, LTg, TR2
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000125735
Entrez:   8740
Chromosome Mapping:   19p13.3

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