PrimePCR™ Probe Assay: PSMB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSMB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSMB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039224
Assay Design:   exonic
Chromosome Location:   1:36068504-36068649question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000373237

Gene Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family also known as the T1B family that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   PSMB2
Gene Name:   proteasome (prosome, macropain) subunit, beta type, 2
Aliases:   HC7-I, MGC104215, MGC126885
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000126067
Entrez:   5690
Chromosome Mapping:   1p34.2

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