PrimePCR™ Probe Assay: PAX3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PAX3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PAX3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039287
Assay Design:   exonic
Chromosome Location:   2:223163366-223163455question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000392069 ENST00000344493 ENST00000350526 ENST00000392070 ENST00000336840 ENST00000409551 ENST00000409828 ENST00000258387

Gene Information

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome craniofacial-deafness-hand syndrome and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14) which represents a fusion between PAX3 and the forkhead gene is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq Jul 2008]

Gene Symbol:   PAX3
Gene Name:   paired box 3
Aliases:   CDHS, HUP2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, WS1, WS3
RefSeq:   NC_000002.11 NG_021186.1 NT_005403.17 NG_011632.1
Ensembl:   ENSG00000135903
Entrez:   5077
Chromosome Mapping:   2q35-q37,2q35

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