PrimePCR™ Probe Assay: RBM15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RBM15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RBM15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039423
Assay Design:   exonic
Chromosome Location:   1:110884168-110884280question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000369784 ENST00000602849 ENST00000487146

Gene Information

Members of the SPEN (Split-end) family of proteins including RBM15 have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al. 2005 [PubMed 16129689]).[supplied by OMIM Feb 2009]

Gene Symbol:   RBM15
Gene Name:   RNA binding motif protein 15
Aliases:   FLJ12479, FLJ21943, MGC119584, OTT, OTT1, SPEN
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000162775
Entrez:   64783
Chromosome Mapping:   1p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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