PrimePCR™ Probe Assay: UGT2A3, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: UGT2A3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: UGT2A3, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0039812
Assay Design:		exonic
Chromosome Location:		4:69795651-69795769question
Amplicon Length:		89
Splice Variants Targeted:		ENST00000551239 ENST00000549514 ENST00000547910 ENST00000373450 ENST00000344644 ENST00000354728 ENST00000373426 ENST00000373424 ENST00000305139 ENST00000373414 ENST00000373409 ENST00000482026 ENST00000305208 ENST00000305231 ENST00000508661 ENST00000446444 ENST00000335568 ENST00000511240 ENST00000512583 ENST00000305107 ENST00000381096 ENST00000503640 ENST00000512704 ENST00000514019 ENST00000286604 ENST00000457664 ENST00000256194 ENST00000527546 ENST00000537344 ENST00000342902 ENST00000379612 ENST

Gene Information

Description Not Available

Gene Symbol:		UGT2A3
Gene Name:		UDP glucuronosyltransferase 2 family, polypeptide A3
Aliases:		FLJ21934
RefSeq:		NC_000004.11 NT_022778.16 NT_167250.1
Ensembl:		ENSG00000135220
Entrez:		79799
Chromosome Mapping:		4q13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.340000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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