PrimePCR™ Probe Assay: DEFB103A, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: DEFB103A, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: DEFB103A, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0039885
Assay Design:		exonic
Chromosome Location:		8:7738790-7738935question
Amplicon Length:		116
Splice Variants Targeted:		ENST00000416252 ENST00000421932 ENST00000419062 ENST00000551081 ENST00000550363 ENST00000447648 ENST00000379795 ENST00000542604 ENST00000314357 ENST00000383362 ENST00000428349 ENST00000383367 ENST00000548973 ENST00000547984 ENST00000416192 ENST00000399074 ENST00000422950 ENST00000399088 ENST00000399065 ENST00000413089 ENST00000469372 ENST00000497706 ENST00000452323 ENST00000299367 ENST00000442278 ENST00000383177 ENST00000378251 ENST00000556679 ENST00000439140 ENST00000456570 ENST00000318124 ENST

Gene Information

Description Not Available

Gene Symbol:		DEFB103A
Gene Name:		defensin, beta 103A
Aliases:		BD-3, DEFB-3, DEFB103, DEFB3, HBD3, HBP-3, HBP3, hBD-3
RefSeq:		NC_000008.10 NT_077531.4
Ensembl:		ENSG00000176797
Entrez:		414325
Chromosome Mapping:		8p23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.330000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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