PrimePCR™ Probe Assay: CCL4L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCL4L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCL4L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040098
Assay Design:   exonic
Chromosome Location:   17:34538527-34539180question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000218104 ENST00000250151 ENST00000394495 ENST00000378350 ENST00000389068 ENST00000588929 ENST00000589079 ENST00000589336 ENST00000400702 ENST00000591167 ENST00000586598 ENST00000591637 ENST00000444414 ENST00000378352 ENST00000358756 ENST00000394465 ENST00000339270 ENST00000482104 ENST00000394463 ENST00000378342 ENST00000368933 ENST00000368930 ENST00000379554 ENST00000379556 ENST00000528567 ENST00000530161 ENST00000525218 ENST00000424273 ENST00000567608 ENST00000562236 ENST00000567807 ENST

Gene Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals; most individuals have 1-5 copies in the diploid genome although rare individuals do not contain this gene at all. The human genome reference assembly contains two copies of this gene. This record represents the more centromeric gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CCL4L1
Gene Name:   chemokine (C-C motif) ligand 4-like 1
Aliases:   AT744.2, CCL4L, CCL4L2, LAG-1, LAG1, SCYA4L
RefSeq:   NC_000017.10 NG_004113.1 NT_010799.15 NW_003315949.1
Ensembl:   ENSG00000205020
Entrez:   9560
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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