PrimePCR™ Probe Assay: DDAH2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: DDAH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DDAH2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040119
Assay Design:   exonic
Chromosome Location:   6:31696423-31696512question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000446426 ENST00000427126 ENST00000426149 ENST00000434464 ENST00000457271 ENST00000434279 ENST00000413287 ENST00000413655 ENST00000424790 ENST00000447101 ENST00000449216 ENST00000427351 ENST00000437288 ENST00000375789 ENST00000375787 ENST00000375792 ENST00000416410 ENST00000436437 ENST00000413343 ENST00000414455 ENST00000444699 ENST00000454138 ENST00000441011 ENST00000417274 ENST00000450563 ENST00000451411 ENST00000430482 ENST00000437889 ENST00000417916 ENST00000437358 ENST00000436457 ENST

Gene Information

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines which in turn inhibit nitric oxide synthase activity. [provided by RefSeq Jul 2008]

Gene Symbol:   DDAH2
Gene Name:   dimethylarginine dimethylaminohydrolase 2
Aliases:   DDAH, DDAHII, G6a, NG30
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000213722
Entrez:   23564
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.150000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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