PrimePCR™ Probe Assay: PLEKHM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLEKHM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLEKHM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040147
Assay Design:   exonic
Chromosome Location:   17:43552770-43552898question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000263035 ENST00000422785 ENST00000299564 ENST00000562812 ENST00000563890 ENST00000566173 ENST00000241124 ENST00000400066 ENST00000400065 ENST00000356192 ENST00000295992 ENST00000487099 ENST00000377603 ENST00000533052 ENST00000443411 ENST00000399138 ENST00000372811 ENST00000372809 ENST00000468090 ENST00000291565 ENST00000467908 ENST00000318442 ENST00000487519 ENST00000417271 ENST00000025008 ENST00000435644 ENST00000518710 ENST00000430334 ENST00000584420 ENST00000589780 ENST00000421073 ENST

Gene Information

The protein encoded by this gene is essential for bone resorption and may play a critical role in vesicular transport in the osteoclast. Mutations in this gene are associated with autosomal recessive osteopetrosis type 6 (OPTB6). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   PLEKHM1
Gene Name:   pleckstrin homology domain containing, family M (with RUN domain) member 1
Aliases:   AP162, B2, KIAA0356, OPTB6
RefSeq:   NC_000017.10 NG_012932.1 NT_010783.15 NT_167251.1
Ensembl:   ENSG00000225190
Entrez:   9842
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.760000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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