PrimePCR™ Probe Assay: HLA-DPA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HLA-DPA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DPA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040176
Assay Design:   exonic
Chromosome Location:   6:33036858-33036951question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000216160 ENST00000515317 ENST00000453314 ENST00000515346 ENST00000512750 ENST00000550456 ENST00000353332 ENST00000296003 ENST00000362057 ENST00000443117 ENST00000419452 ENST00000447657 ENST00000439571 ENST00000551196 ENST00000419277 ENST00000437811 ENST00000453337 ENST00000428995 ENST00000390649 ENST00000400335 ENST00000400338 ENST00000367602 ENST00000454805 ENST00000442259 ENST00000437676 ENST00000436183 ENST00000549300 ENST00000374808 ENST00000429960 ENST00000441306 ENST00000414177 ENST

Gene Information

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities resulting in up to 4 different molecules. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DPA1
Gene Name:   major histocompatibility complex, class II, DP alpha 1
Aliases:   HLA-DP1A, HLADP, HLASB, PLT1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000231389
Entrez:   3113
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.430000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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