PrimePCR™ Probe Assay: B3GALT4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: B3GALT4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: B3GALT4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040196
Assay Design:   exonic
Chromosome Location:   6:33246284-33246394question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000415322 ENST00000383209 ENST00000451237 ENST00000419471 ENST00000430971

Gene Information

This gene is a member of the beta-13-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine galactose N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1 whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3 beta3GalT5). This gene is oriented telomere to centromere in close proximity to the ribosomal protein S18 gene. The functionality of the encoded protein is limited to ganglioseries glycolipid biosynthesis. [provided by RefSeq Jul 2008]

Gene Symbol:   B3GALT4
Gene Name:   UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
Aliases:   BETA3GALT4, GALT2, GALT4
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000235863
Entrez:   8705
Chromosome Mapping:   6p21.3

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