PrimePCR™ Probe Assay: HLA-DOB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HLA-DOB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DOB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040215
Assay Design:   exonic
Chromosome Location:   6:32782945-32783079question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000426644 ENST00000453971 ENST00000420764 ENST00000395550 ENST00000383733 ENST00000337000 ENST00000455886 ENST00000358747 ENST00000564819 ENST00000544589 ENST00000454286 ENST00000413252 ENST00000454969 ENST00000448690 ENST00000447178 ENST00000439310 ENST00000456406 ENST00000455155 ENST00000527042 ENST00000366663 ENST00000470540 ENST00000427697 ENST00000523410 ENST00000438763 ENST00000447394

Gene Information

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB) both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DOB
Gene Name:   major histocompatibility complex, class II, DO beta
Aliases:   DOB, FLJ57033
RefSeq:   NC_000006.11 NG_012008.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167249.1
Ensembl:   ENSG00000241106
Entrez:   3112
Chromosome Mapping:   6p21.3

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