PrimePCR™ Probe Assay: RP11-548K23.11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: RP11-548K23.11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-548K23.11, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040253
Assay Design:   exonic
Chromosome Location:   10:99344292-99344467question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000370646 ENST00000370647 ENST00000555577 ENST00000264926 ENST00000370649 ENST00000338110 ENST00000395248 ENST00000566897 ENST00000569545 ENST00000563060 ENST00000412304 ENST00000564546 ENST00000564595 ENST00000569798 ENST00000395240 ENST00000563311 ENST00000563654 ENST00000568960 ENST00000566765 ENST00000569567 ENST00000360835 ENST00000290460 ENST00000281928 ENST00000354649 ENST00000397802 ENST00000526683 ENST00000453551 ENST00000313352 ENST00000456095 ENST00000349157 ENST00000203556 ENST

Gene Information

Description Not Available

Gene Symbol:   RP11-548K23.11
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000249967
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.440000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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