PrimePCR™ Probe Assay: CTD-2135J3.4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CTD-2135J3.4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTD-2135J3.4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040281
Assay Design:   exonic
Chromosome Location:   8:10582929-10583071question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000539371 ENST00000304501 ENST00000553390 ENST00000554914 ENST00000264951 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST00000469640 ENST00000430121 ENST00000465829 ENST00000481893 ENST00000306682 ENST00000541662 ENST00000262940 ENST00000461209 ENST00000449970 ENST00000462172 ENST00000522801 ENST00000541884

Gene Information

Description Not Available

Gene Symbol:   CTD-2135J3.4
Gene Name:   SRY (sex determining region Y)-box 7; Uncharacterized protein; cDNA FLJ58508, highly similar to Transcription factor SOX-7
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000258724
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.400000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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