PrimePCR™ Probe Assay: VAV3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VAV3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VAV3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040336
Assay Design:   exonic
Chromosome Location:   1:108185259-108226126question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000370056 ENST00000527011 ENST00000415432 ENST00000529809 ENST00000544443

Gene Information

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG RhoA and to a lesser extent RAC1 and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   VAV3
Gene Name:   vav 3 guanine nucleotide exchange factor
Aliases:   FLJ40431
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000134215
Entrez:   10451
Chromosome Mapping:   1p13.3

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