PrimePCR™ Probe Assay: NDUFB6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NDUFB6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NDUFB6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040409
Assay Design:   exonic
Chromosome Location:   9:32573027-32573142question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000379847 ENST00000350021

Gene Information

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Jan 2011]

Gene Symbol:   NDUFB6
Gene Name:   NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa
Aliases:   B17, CI, MGC13675
RefSeq:   NG_017050.1 NC_000009.11 NT_008413.18 NG_011511.1
Ensembl:   ENSG00000165264
Entrez:   4712
Chromosome Mapping:   9p21.1

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