PrimePCR™ Probe Assay: HIF3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best negative control

List Price:    $263.00
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PrimePCR™ PreAmp for Probe Assay: HIF3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HIF3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040499
Assay Design:   exonic
Chromosome Location:   19:46812498-46815427question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000377670 ENST00000244303 ENST00000472815 ENST00000300862 ENST00000600383 ENST00000420102 ENST00000339613

Gene Information

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   HIF3A
Gene Name:   hypoxia inducible factor 3, alpha subunit
Aliases:   HIF-3A, IPAS, MOP7, PASD7, bHLHe17
RefSeq:   NC_000019.9 NG_028163.1 NT_011109.16
Ensembl:   ENSG00000124440
Entrez:   64344
Chromosome Mapping:   19q13.32

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