PrimePCR™ Probe Assay: SPOCK3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SPOCK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPOCK3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040557
Assay Design:   exonic
Chromosome Location:   4:167675744-167675847question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000357154 ENST00000357545 ENST00000504953 ENST00000511531 ENST00000506886 ENST00000510741 ENST00000502330 ENST00000512681 ENST00000511269 ENST00000512648 ENST00000510403 ENST00000541637 ENST00000534949 ENST00000421836 ENST00000535728 ENST00000541354

Gene Information

This gene encodes a member of a novel family of calcium-binding proteoglycan proteins that contain thyroglobulin type-1 and Kazal-like domains. The encoded protein and may play a role in adult T-cell leukemia by inhibiting the activity of membrane-type matrix metalloproteinases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   SPOCK3
Gene Name:   sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
Aliases:   HSAJ1454, TES-3
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000196104
Entrez:   50859
Chromosome Mapping:   4q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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