PrimePCR™ Probe Assay: DIO1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DIO1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DIO1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040635
Assay Design:   exonic
Chromosome Location:   1:54370339-54370445question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000529589 ENST00000361921 ENST00000322679 ENST00000525202 ENST00000524406

Gene Information

The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some but not all isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq Jul 2008]

Gene Symbol:   DIO1
Gene Name:   deiodinase, iodothyronine, type I
Aliases:   5DI, MGC130050, MGC130051, TXDI1
RefSeq:   NC_000001.10 NG_023306.1 NT_032977.9
Ensembl:   ENSG00000211452
Entrez:   1733
Chromosome Mapping:   1p33-p32

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