PrimePCR™ Probe Assay: HFE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HFE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HFE, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040640
Assay Design:   exonic
Chromosome Location:   6:26092948-26093070question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000397022 ENST00000353147 ENST00000349999 ENST00000317896 ENST00000357618 ENST00000470149 ENST00000336625 ENST00000461397 ENST00000488199 ENST00000309234 ENST00000360319 ENST00000369850 ENST00000422373 ENST00000369856 ENST00000346618 ENST00000535432 ENST00000596447 ENST00000597475 ENST00000600520 ENST00000594381

Gene Information

The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder hereditary haemochromatosis is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HFE
Gene Name:   hemochromatosis
Aliases:   HFE1, HH, HLA-H, MGC103790, MVCD7, dJ221C16.10.1
RefSeq:   NC_000006.11 NG_001335.1 NG_008720.1 NT_007592.15
Ensembl:   ENSG00000010704
Entrez:   3077
Chromosome Mapping:   6p21.3

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