PrimePCR™ Probe Assay: H2AFV, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: H2AFV, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: H2AFV, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040693
Assay Design:   exonic
Chromosome Location:   7:44875139-44875233question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000446531 ENST00000350771 ENST00000308153 ENST00000349299 ENST00000437072 ENST00000222690

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   H2AFV
Gene Name:   H2A histone family, member V
Aliases:   FLJ26479, H2AV, MGC10170, MGC10831, MGC1947
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000105968
Entrez:   94239
Chromosome Mapping:   7p13

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