PrimePCR™ Probe Assay: OGFOD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: OGFOD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: OGFOD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040707
Assay Design:   exonic
Chromosome Location:   12:123463708-123463802question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000529859 ENST00000529619 ENST00000519716 ENST00000428389 ENST00000252773 ENST00000418814 ENST00000505769 ENST00000457062 ENST00000361499 ENST00000505868 ENST00000370479 ENST00000380985 ENST00000502464 ENST00000260585 ENST00000301067 ENST00000542678 ENST00000397389 ENST00000538755 ENST00000536150 ENST00000545056 ENST00000545612 ENST00000538628 ENST00000545317 ENST00000454694 ENST00000228922 ENST00000536439 ENST00000542027 ENST00000508956 ENST00000423778 ENST00000503669 ENST00000310653 ENST

Gene Information

Description Not Available

Gene Symbol:   OGFOD2
Gene Name:   2-oxoglutarate and iron-dependent oxygenase domain containing 2
Aliases:   DKFZp686H15154, FLJ13491, FLJ37501, MGC120434, MGC120436
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000111325
Entrez:   79676
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.560000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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