PrimePCR™ Probe Assay: SORBS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SORBS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SORBS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040723
Assay Design:   exonic
Chromosome Location:   8:22424627-22426690question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000263246 ENST00000407585 ENST00000403744 ENST00000402229 ENST00000337959 ENST00000529750 ENST00000529432 ENST00000240123 ENST00000523900 ENST00000518912 ENST00000523965 ENST00000521554 ENST00000522721 ENST00000523348 ENST00000428103 ENST00000266560 ENST00000262483 ENST00000367155 ENST00000304076 ENST00000602142 ENST00000596764 ENST00000599806 ENST00000539284 ENST00000323110 ENST00000528956 ENST00000372492 ENST00000589082

Gene Information

This gene encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization cell adhesion and migration signaling and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   SORBS3
Gene Name:   sorbin and SH3 domain containing 3
Aliases:   SCAM-1, SCAM1, SH3D4, VINEXIN
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000120896
Entrez:   10174
Chromosome Mapping:   8p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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