PrimePCR™ Probe Assay: RABL2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RABL2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: RABL2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040795
Assay Design:   exonic
Chromosome Location:   2:114386153-114386265question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000244364 ENST00000370788 ENST00000421834 ENST00000393167 ENST00000409842 ENST00000413545 ENST00000393165 ENST00000393166 ENST00000409875 ENST00000376439 ENST00000317089 ENST00000276297 ENST00000316609 ENST00000511869 ENST00000287701 ENST00000397358 ENST00000444075 ENST00000354869 ENST00000395593 ENST00000395598 ENST00000395591 ENST00000395595 ENST00000395590 ENST00000435118 ENST00000599205 ENST00000599617 ENST00000595999 ENST00000594429 ENST00000594223 ENST00000596737 ENST00000598380

Gene Information

This gene is a member of the RAB gene family which belongs to the RAS GTPase superfamily. The proteins in the family of RAS-related signaling molecules are small GTP-binding proteins that play important roles in the regulation of exocytotic and endocytotic pathways. This gene maps to the site of an ancestral telomere fusion event and may be a subtelomeric gene. Two alternatively spliced transcript variants have been identified both encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   RABL2A
Gene Name:   RAB, member of RAS oncogene family-like 2A
Aliases:   FLJ78724, MGC117180
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000144134
Entrez:   11159
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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