PrimePCR™ Probe Assay: DISC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DISC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DISC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040835
Assay Design:   exonic
Chromosome Location:   1:231830381-231830542question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000508064 ENST00000311573 ENST00000368340 ENST00000347088 ENST00000361831 ENST00000355499 ENST00000404643 ENST00000359205 ENST00000407221 ENST00000295566 ENST00000368330 ENST00000368339 ENST00000535662 ENST00000317586 ENST00000539444 ENST00000602281 ENST00000366633 ENST00000366636 ENST00000366637 ENST00000439617 ENST00000535983 ENST00000537876

Gene Information

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   DISC1
Gene Name:   disrupted in schizophrenia 1
Aliases:   C1orf136, FLJ13381, FLJ21640, FLJ25311, FLJ41105, KIAA0457, SCZD9
RefSeq:   NC_000001.10 NT_167186.1 NG_011681.1
Ensembl:   ENSG00000162946
Entrez:   27185
Chromosome Mapping:   1q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.740000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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