PrimePCR™ Probe Assay: AQP7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AQP7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AQP7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040847
Assay Design:   exonic
Chromosome Location:   9:33385726-33386076question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000544264 ENST00000164024 ENST00000561882 ENST00000202028 ENST00000373950 ENST00000338074 ENST00000373941 ENST00000441639 ENST00000285238 ENST00000288502 ENST00000262738 ENST00000371564 ENST00000396009 ENST00000414705 ENST00000320785 ENST00000447660 ENST00000379507 ENST00000297988 ENST00000439678 ENST00000379506 ENST00000379503 ENST00000377425 ENST00000541274 ENST00000539936 ENST00000537089 ENST00000540393 ENST00000254695 ENST00000366401 ENST00000542807 ENST00000291503 ENST00000357324 ENST

Gene Information

Aquaporins/major intrinsic protein (MIP) are a family of water-selective membrane channels. Aquaporin 7 has greater sequence similarity with AQP3 and AQP9 and they may be a subfamily. Aquaporin 7 and AQP3 are at the same chromosomal location suggesting that 9p13 may be a site of an aquaporin cluster. Aquaporin 7 facilitates water glycerol and urea transport. It may play an important role in sperm function. [provided by RefSeq Jul 2008]

Gene Symbol:   AQP7
Gene Name:   aquaporin 7
Aliases:   AQP7L, AQP9, AQPap, MGC149555, MGC149556
RefSeq:   NC_000009.11 NG_027764.1 NT_008413.18
Ensembl:   ENSG00000165269
Entrez:   364
Chromosome Mapping:   9p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.600000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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