PrimePCR™ Probe Assay: XAGE3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: XAGE3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: XAGE3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040887
Assay Design:   exonic
Chromosome Location:   X:52895473-52895566question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000323372 ENST00000375501 ENST00000351072 ENST00000425386 ENST00000375503 ENST00000375491 ENST00000346279 ENST00000374951 ENST00000519203 ENST00000260283 ENST00000524756 ENST00000528829 ENST00000533353 ENST00000527598 ENST00000357139 ENST00000305444 ENST00000593744 ENST00000601552 ENST00000600900 ENST00000594956 ENST00000598948 ENST00000601427

Gene Information

This gene is a member of the XAGE subfamily which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is expressed in placenta and fetal liver/spleen and may function in inhibiting cancer cell growth. The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 2 transcript variants differing in the 5' UTR. [provided by RefSeq Jul 2008]

Gene Symbol:   XAGE3
Gene Name:   X antigen family, member 3
Aliases:   CT12.3a, CT12.3b, GAGED4, MGC71925, PLAC6, XAGE-3, pp9012
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000171402
Entrez:   170626
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.050000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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