PrimePCR™ Probe Assay: RPL15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RPL15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPL15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040906
Assay Design:   exonic
Chromosome Location:   3:23959355-23959450question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000307839 ENST00000422218 ENST00000434031 ENST00000413699 ENST00000412097 ENST00000415719 ENST00000435882 ENST00000354811 ENST00000456530

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10 its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Nov 2011]

Gene Symbol:   RPL15
Gene Name:   ribosomal protein L15
Aliases:   EC45, FLJ26304, L15, MGC88603, RPL10, RPLY10, RPYL10
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000174748
Entrez:   6138
Chromosome Mapping:   3p24.2

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