PrimePCR™ Probe Assay: ARL6IP4, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: ARL6IP4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: ARL6IP4, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0040938
Assay Design:		exonic
Chromosome Location:		12:123466504-123466593question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000339591 ENST00000584939 ENST00000449880 ENST00000450894 ENST00000200181 ENST00000579662 ENST00000356166 ENST00000578147 ENST00000435607 ENST00000525621 ENST00000529370 ENST00000264818 ENST00000400068 ENST00000356025 ENST00000358771 ENST00000371222 ENST00000307169 ENST00000264930 ENST00000552421 ENST00000325680 ENST00000238571 ENST00000543566 ENST00000315580 ENST00000542099 ENST00000392435 ENST00000413381 ENST00000426960 ENST00000453766 ENST00000454885 ENST00000439686 ENST00000456762 ENST

Gene Information

Description Not Available

Gene Symbol:		ARL6IP4
Gene Name:		ADP-ribosylation-like factor 6 interacting protein 4
Aliases:		MGC814, SFRS20, SR-25, SRp25, SRrp37
RefSeq:		NC_000012.11 NT_009755.19
Ensembl:		ENSG00000182196
Entrez:		51329
Chromosome Mapping:		12q24.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	36.030000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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