PrimePCR™ Probe Assay: PDE6G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PDE6G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDE6G, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040960
Assay Design:   exonic
Chromosome Location:   17:79618075-79618678question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000574024 ENST00000573076 ENST00000571224 ENST00000571004 ENST00000331056 ENST00000568954 ENST00000393524 ENST00000356272

Gene Information

This gene encodes the gamma subunit of cyclic GMP-phosphodiesterase which is composed of alpha- and beta- catalytic subunits and two identical inhibitory gamma subunits. This gene is expressed in rod photoreceptors and functions in the phototransduction signaling cascade. It is also expressed in a variety of other tissues and has been shown to regulate the c-Src protein kinase and G-protein-coupled receptor kinase 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2009]

Gene Symbol:   PDE6G
Gene Name:   phosphodiesterase 6G, cGMP-specific, rod, gamma
Aliases:   DKFZp686C0587, MGC125749, PDEG, RP57
RefSeq:   NC_000017.10 NG_009834.1 NT_010783.15
Ensembl:   ENSG00000185527
Entrez:   5148
Chromosome Mapping:   17q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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