PrimePCR™ Probe Assay: ATP6V0C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP6V0C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP6V0C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040965
Assay Design:   exonic
Chromosome Location:   16:2569274-2569559question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000564543 ENST00000330398 ENST00000568562 ENST00000565223 ENST00000564973 ENST00000326181 ENST00000367674 ENST00000263525

Gene Information

This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c" and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17. [provided by RefSeq Nov 2010]

Gene Symbol:   ATP6V0C
Gene Name:   ATPase, H+ transporting, lysosomal 16kDa, V0 subunit c
Aliases:   ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000185883
Entrez:   527
Chromosome Mapping:   16p13.3

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