PrimePCR™ Probe Assay: PRG2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PRG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRG2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040971
Assay Design:   exonic
Chromosome Location:   11:57156531-57156685question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000551473 ENST00000221972 ENST00000311862 ENST00000533605 ENST00000525955 ENST00000529411 ENST00000361475 ENST00000536724 ENST00000338380 ENST00000396432 ENST00000376410 ENST00000446003 ENST00000320481 ENST00000431670 ENST00000498699 ENST00000412622 ENST00000261800 ENST00000465829 ENST00000541662 ENST00000262940 ENST00000461209 ENST00000449970 ENST00000261381

Gene Information

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA) angiotensinogen (AGT) and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage exfoliation and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   PRG2
Gene Name:   proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
Aliases:   BMPG, MBP, MBP1, MGC14537
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000186652
Entrez:   5553
Chromosome Mapping:   11q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.540000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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