PrimePCR™ Probe Assay: SRSF10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SRSF10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRSF10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040983
Assay Design:   exonic
Chromosome Location:   1:24298390-24301466question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000344989 ENST00000453840 ENST00000484146 ENST00000374452 ENST00000374453 ENST00000343255 ENST00000492112 ENST00000433682 ENST00000374457 ENST00000374449

Gene Information

This gene product is a member of the serine-arginine (SR) family of proteins which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs which are required for binding to RNA and multiple C-terminal SR/RS repeats which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS and abrogates the influence of TLS on E1A pre-mRNA splicing. This gene has multiple pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. In addition transcript variants utilizing alternative polyA sites exist.[provided by RefSeq Jul 2010]

Gene Symbol:   SRSF10
Gene Name:   serine/arginine-rich splicing factor 10
Aliases:   DKFZp686H0644, FLJ30749, FLJ43846, FUSIP1, FUSIP2, NSSR, SFRS13, SFRS13A, SRp38, SRrp40, TASR, TASR1, TASR2
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000188529
Entrez:   10772
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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