PrimePCR™ Probe Assay: SRSF10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SRSF10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRSF10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040983
Assay Design:   exonic
Chromosome Location:   1:24298390-24301466question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000344989 ENST00000453840 ENST00000484146 ENST00000374452 ENST00000374453 ENST00000343255 ENST00000492112 ENST00000433682 ENST00000374457 ENST00000374449

Gene Information

This gene product is a member of the serine-arginine (SR) family of proteins which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs which are required for binding to RNA and multiple C-terminal SR/RS repeats which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS and abrogates the influence of TLS on E1A pre-mRNA splicing. This gene has multiple pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. In addition transcript variants utilizing alternative polyA sites exist.[provided by RefSeq Jul 2010]

Gene Symbol:   SRSF10
Gene Name:   serine/arginine-rich splicing factor 10
Aliases:   DKFZp686H0644, FLJ30749, FLJ43846, FUSIP1, FUSIP2, NSSR, SFRS13, SFRS13A, SRp38, SRrp40, TASR, TASR1, TASR2
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000188529
Entrez:   10772
Chromosome Mapping:   1p36.11

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