PrimePCR™ Probe Assay: HBG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HBG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HBG1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041095
Assay Design:   exonic
Chromosome Location:   11:5269533-5269650question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000330597 ENST00000380252 ENST00000380259 ENST00000336906 ENST00000355947 ENST00000349062 ENST00000373855 ENST00000373850 ENST00000431571 ENST00000238341 ENST00000327325 ENST00000595751 ENST00000596707 ENST00000597522 ENST00000595496 ENST00000394122 ENST00000248228 ENST00000359059 ENST00000357361 ENST00000205557

Gene Information

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq Jul 2008]

Gene Symbol:   HBG1
Gene Name:   hemoglobin, gamma A
Aliases:   HBGA, HBGR, HSGGL1
RefSeq:   NC_000011.9 NG_000007.3 NT_009237.18
Ensembl:   ENSG00000213934
Entrez:   3047
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.190000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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