PrimePCR™ Probe Assay: SLC35E2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: SLC35E2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC35E2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041106
Assay Design:   exonic
Chromosome Location:   1:1670765-1670871question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000346932 ENST00000350527 ENST00000537485 ENST00000322049 ENST00000380378 ENST00000378662 ENST00000234800 ENST00000319153 ENST00000355439 ENST00000246421 ENST00000400924 ENST00000361661 ENST00000354546 ENST00000523714 ENST00000377751 ENST00000521512 ENST00000356496 ENST00000534749 ENST00000436757 ENST00000356404 ENST00000359591 ENST00000546834 ENST00000551466 ENST00000443128 ENST00000219299 ENST00000523094 ENST00000530742 ENST00000411809 ENST00000523908 ENST00000521615 ENST00000296951 ENST

Gene Information

Description Not Available

Gene Symbol:   SLC35E2
Gene Name:   solute carrier family 35, member E2
Aliases:   DKFZp686M0869, FLJ34996, FLJ44537, KIAA0447, MGC104754, MGC117254, MGC126715, MGC138494
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000215790
Entrez:   9906
Chromosome Mapping:   1p36.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.230000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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