PrimePCR™ Probe Assay: CKLF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CKLF, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CKLF, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041109
Assay Design:   exonic
Chromosome Location:   16:66586546-66586693question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000264001 ENST00000351137 ENST00000345436 ENST00000362093 ENST00000417030 ENST00000532838 ENST00000527729

Gene Information

The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CMTM1 (CKLF-like MARVEL transmembrane domain containing 1).[provided by RefSeq Feb 2011]

Gene Symbol:   CKLF
Gene Name:   chemokine-like factor
Aliases:   C32, CKLF1, CKLF2, CKLF3, CKLF4, UCK-1
RefSeq:   NC_000016.9 NG_016862.1 NT_010498.15
Ensembl:   ENSG00000217555
Entrez:   51192
Chromosome Mapping:   16q21

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