PrimePCR™ Probe Assay: PLA2G4B, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: PLA2G4B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: PLA2G4B, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0041163
Assay Design:		exonic
Chromosome Location:		15:42137046-42137262question
Amplicon Length:		187
Splice Variants Targeted:		ENST00000418259 ENST00000377264 ENST00000421419 ENST00000430939 ENST00000569188 ENST00000512215 ENST00000394854 ENST00000323055 ENST00000394853 ENST00000507176 ENST00000523694 ENST00000587303 ENST00000592099 ENST00000588012 ENST00000372412 ENST00000357368 ENST00000262963 ENST00000348075 ENST00000353284 ENST00000261722 ENST00000535348 ENST00000535359 ENST00000342115 ENST00000528667 ENST00000256578 ENST00000358729 ENST00000369840 ENST00000528454 ENST00000393688 ENST00000382448 ENST00000342159 ENST

Gene Information

Description Not Available

Gene Symbol:		PLA2G4B
Gene Name:		phospholipase A2, group IVB (cytosolic)
Aliases:		DKFZp686N12131, FLJ17693, FLJ42498, FLJ53173, HsT16992, cPLA2-beta
RefSeq:		NC_000015.9 NT_010194.17
Ensembl:		ENSG00000243708
Entrez:		100137049
Chromosome Mapping:		15q11.2-q21.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	36.810000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
10000143205	SARS-CoV-2 Variant Detection RT-PCR Assay Protocol	Click to download
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