PrimePCR™ Probe Assay: ZFX, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZFX, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZFX, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041245
Assay Design:   exonic
Chromosome Location:   X:24229453-24229572question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000379177 ENST00000304543 ENST00000338565 ENST00000379188 ENST00000540034 ENST00000539115

Gene Information

This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD) a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified but the full-length nature of some variants has not been determined. [provided by RefSeq May 2010]

Gene Symbol:   ZFX
Gene Name:   zinc finger protein, X-linked
Aliases:   ZNF926
RefSeq:   NC_000023.10 NT_167197.1 NG_021253.1
Ensembl:   ENSG00000005889
Entrez:   7543
Chromosome Mapping:   Xp21.3

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