PrimePCR™ Probe Assay: POU5F1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POU5F1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POU5F1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041249
Assay Design:   exonic
Chromosome Location:   8:128427859-128428008question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000465342 ENST00000391675

Gene Information

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1 however it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq Apr 2009]

Gene Symbol:   POU5F1B
Gene Name:   POU class 5 homeobox 1B
Aliases:   OCT4-PG1, OTF3C, OTF3P1, POU5F1P1, POU5F1P4, POU5FLC20, POU5FLC8
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000212993
Entrez:   5462
Chromosome Mapping:   8q24.21

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