PrimePCR™ Probe Assay: HS3ST1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HS3ST1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HS3ST1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041354
Assay Design:   exonic
Chromosome Location:   4:11401424-11401531question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000293328 ENST00000345506 ENST00000590949 ENST00000546010 ENST00000588868 ENST00000452307 ENST00000399848 ENST00000359446 ENST00000361205 ENST00000402119 ENST00000002596 ENST00000514690 ENST00000510712 ENST00000253083 ENST00000450961 ENST00000288699 ENST00000401478 ENST00000431402 ENST00000434719 ENST00000280481 ENST00000378156 ENST00000360469 ENST00000399953 ENST00000262313 ENST00000399951 ENST00000262429 ENST00000416219 ENST00000360986 ENST00000315782 ENST00000382480 ENST00000429646 ENST

Gene Information

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity anticoagulant heparan sulfate conversion activity and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq Jul 2008]

Gene Symbol:   HS3ST1
Gene Name:   heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Aliases:   3OST, 3OST1
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000002587
Entrez:   9957
Chromosome Mapping:   4p16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.220000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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