PrimePCR™ Probe Assay: SNRPN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SNRPN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNRPN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041415
Assay Design:   exonic
Chromosome Location:   15:25220525-25221461question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000400100 ENST00000400097 ENST00000390687 ENST00000584968 ENST00000346403 ENST00000554227 ENST00000579070 ENST00000577565 ENST00000400098 ENST00000444203 ENST00000338094

Gene Information

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. [provided by RefSeq Jul 2008]

Gene Symbol:   SNRPN
Gene Name:   small nuclear ribonucleoprotein polypeptide N
Aliases:   DKFZp686C0927, DKFZp686M12165, DKFZp761I1912, DKFZp762N022, FLJ33569, FLJ36996, FLJ39265, HCERN3, MGC29886, PWCR, RT-LI, SM-D, SMN, SNRNP-N, SNURF-SNRPN
RefSeq:   NG_009268.1 NG_021193.1 NG_021436.1 NC_000015.9 NG_002690.1 NT_026446.14 NG_012958.1
Ensembl:   ENSG00000128739
Entrez:   6638
Chromosome Mapping:   15q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.710000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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