PrimePCR™ Probe Assay: RAPGEF6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAPGEF6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAPGEF6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041446
Assay Design:   exonic
Chromosome Location:   5:130846089-130857100question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000598766 ENST00000376251 ENST00000323022 ENST00000376265 ENST00000540582 ENST00000262519 ENST00000591462 ENST00000592741 ENST00000587327 ENST00000589838 ENST00000252455 ENST00000412601 ENST00000429576 ENST00000509018 ENST00000507093 ENST00000296859 ENST00000308008 ENST00000510071 ENST00000513227 ENST00000504575 ENST00000504039 ENST00000307984 ENST00000514667 ENST00000358517 ENST00000367328 ENST00000573439 ENST00000360565 ENST00000601604 ENST00000598102 ENST00000599684

Gene Information

Description Not Available

Gene Symbol:   RAPGEF6
Gene Name:   Rap guanine nucleotide exchange factor (GEF) 6
Aliases:   DKFZp667N084, DKFZp686I15116, KIA001LB, PDZ-GEF2, PDZGEF2, RA-GEF-2, RAGEF2
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000158987
Entrez:   51735
Chromosome Mapping:   5q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995100
y-intercept 34.670000
Efficiency 108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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