PrimePCR™ Probe Assay: CCS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CCS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041477
Assay Design:   exonic
Chromosome Location:   11:66372812-66372974question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000602628 ENST00000264661 ENST00000293328 ENST00000345506 ENST00000590949 ENST00000546010 ENST00000588868 ENST00000452307 ENST00000345728 ENST00000279227 ENST00000432854 ENST00000412678 ENST00000259746 ENST00000371893 ENST00000323267 ENST00000370223 ENST00000370220 ENST00000297283 ENST00000593303 ENST00000533589 ENST00000533244 ENST00000310190 ENST00000534763 ENST00000263816 ENST00000245934 ENST00000252032 ENST00000321613 ENST00000454243 ENST00000284110 ENST00000300571 ENST00000535671 ENST

Gene Information

Copper chaperone for superoxide dismutase specifically delivers Cu to copper/zinc superoxide dismutase and may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. [provided by RefSeq Jul 2008]

Gene Symbol:   CCS
Gene Name:   copper chaperone for superoxide dismutase
Aliases:   MGC138260
RefSeq:   NC_000011.9 NG_008733.1 NT_167190.1
Ensembl:   ENSG00000173992
Entrez:   9973
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.860000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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