PrimePCR™ Probe Assay: SFTPA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SFTPA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SFTPA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041496
Assay Design:   exonic
Chromosome Location:   10:81319071-81319204question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000443071 ENST00000556751 ENST00000355173 ENST00000416252 ENST00000550363 ENST00000372325 ENST00000417041 ENST00000492049 ENST00000372327 ENST00000371482 ENST00000370003 ENST00000419470 ENST00000394569 ENST00000429958 ENST00000439264 ENST00000372308 ENST00000398636 ENST00000428376 ENST00000383362 ENST00000547984 ENST00000397708 ENST00000291688 ENST00000393235 ENST00000311085 ENST00000539542 ENST00000373631 ENST00000373629 ENST00000299367 ENST00000418949 ENST00000537055 ENST00000601659 ENST

Gene Information

This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby which affect the highly conserved carbohydrate recognition domain are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. [provided by RefSeq Sep 2009]

Gene Symbol:   SFTPA2
Gene Name:   surfactant protein A2
Aliases:   COLEC5, FLJ50594, FLJ50597, FLJ51953, FLJ79091, FLJ93678, MGC133169, MGC133366, MGC189714, MGC189761, PSAP, SFTP1, SFTPA2B, SPA2, SPAII
RefSeq:   NC_000010.10 NG_013046.1 NT_030059.13
Ensembl:   ENSG00000185303
Entrez:   729238
Chromosome Mapping:   10q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.500000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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