PrimePCR™ Probe Assay: ZNF655, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF655, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF655, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041509
Assay Design:   exonic
Chromosome Location:   7:99158187-99158310question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000320583 ENST00000452314 ENST00000252713 ENST00000454654 ENST00000449244 ENST00000425063 ENST00000493277 ENST00000422164 ENST00000422647 ENST00000427931 ENST00000440391 ENST00000394163 ENST00000424881 ENST00000357864 ENST00000352645 ENST00000351589 ENST00000361204 ENST00000432879 ENST00000430524 ENST00000423738 ENST00000536485 ENST00000574722 ENST00000307630 ENST00000370401 ENST00000262858 ENST00000453996 ENST00000312060 ENST00000567949 ENST00000378384 ENST00000557272 ENST00000597860 ENST

Gene Information

This gene encodes a zinc finger protein. The zinc finger proteins are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF655
Gene Name:   zinc finger protein 655
Aliases:   DKFZp686M1631, FLJ23461, MGC10859, MGC16203, MGC5521, VIK, VIK-1
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000197343
Entrez:   79027
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.110000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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