PrimePCR™ Probe Assay: MYL6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MYL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYL6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041632
Assay Design:   exonic
Chromosome Location:   12:56554027-56554422question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000553066 ENST00000550443 ENST00000552568 ENST00000207437 ENST00000550697 ENST00000548580 ENST00000293422 ENST00000348108 ENST00000549017 ENST00000549566 ENST00000536128 ENST00000547649 ENST00000547408 ENST00000551589 ENST00000549392 ENST00000548400 ENST00000548293

Gene Information

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains two nonphosphorylatable alkali light chains and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain that is expressed in smooth muscle and non-muscle tissues. Genomic sequences representing several pseudogenes have been described and two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MYL6
Gene Name:   myosin, light chain 6, alkali, smooth muscle and non-muscle
Aliases:   ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3NM, MLC3SM
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000092841
Entrez:   4637
Chromosome Mapping:   12q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.300000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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