PrimePCR™ Probe Assay: MAGEA6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAGEA6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGEA6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041696
Assay Design:   exonic
Chromosome Location:   X:151869488-151869586question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000221972 ENST00000430170 ENST00000445164 ENST00000371890 ENST00000447224 ENST00000262577 ENST00000539594 ENST00000289865 ENST00000368002 ENST00000378423 ENST00000353224 ENST00000378429 ENST00000547669 ENST00000549768 ENST00000377670 ENST00000244303 ENST00000300862 ENST00000339613 ENST00000300850 ENST00000394979 ENST00000547056 ENST00000549383 ENST00000537442 ENST00000382421 ENST00000545200 ENST00000399466 ENST00000322166 ENST00000541778 ENST00000199936 ENST00000550609 ENST00000550109 ENST

Gene Information

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders such as dyskeratosis congenita. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   MAGEA6
Gene Name:   melanoma antigen family A, 6
Aliases:   CT1.6, MAGE-3b, MAGE3B, MAGE6, MGC52297
RefSeq:   NC_000023.10 NT_167198.1
Ensembl:   ENSG00000197172
Entrez:   4105
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.750000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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